Incubation:Article Title: Therapeutic Potential of Perillaldehyde in Ameliorating Vulvovaginal Candidiasis by Reducing Vaginal Oxidative Stress and Apoptosis
Article Snippet: .. Blocked PVDF membranes were incubated with the following primary antibodies at 4 °C overnight: Rabbit anti-PARP (CST, Danvers, MA, USA), Rabbit anti-caspase-9 (CST, Danvers, MA, USA), Rabbit anti-caspase-3 (CST, Danvers, MA, USA), Rabbit anti-caspase-7 (CST, Danvers, MA, USA), Mouse anti-caspase-1 (CST, Danvers, MA, USA), Rabbit anti-Bcl-2 (CST, Danvers, MA, USA), Rabbit anti-Bax (CST, Danvers, MA, USA), Rabbit anti-HO-1 (Abways, Shanghai, China), Rabbit anti-NOX-2 (Bioss, Beijing, China), Mouse anti-Keap1 (Bioworld, Nanjing, China), Rabbit anti-Nrf2 (Bioworld, Nanjing, China), Mouse anti-Asc (Santa Cruz, Santa Cruz, CA, USA), Goat anti-NLRP3 (Abcam, Cambridge, MA, USA). .. The next day, after washing with TBST, the membranes were incubated with secondary antibodies: horseradish peroxidase- (HRP-) linked Goat anti-rabbit (CST, Danvers, MA, USA), Donkey anti-goat (Abcam, Cambridge, MA, USA) or Rabbit anti-mouse (CST, Danvers, MA, USA) for 1 h at room temperature on a shaker.
Article Title: A pharmacological modality to sequester homomeric proteins
Article Snippet: 40 μg of each sample was loaded and run on an SDS-PAGE gel, then transferred onto a nitrocellulose membrane using a Trans-Blot Turbo system (Bio-Rad) and blocked using a 5% BSA in TBS-T (w/v) solution for 1 h at room temperature. .. Incubation with primary antibodies (mouse anti-BCL-6 (sc-7388, Santa Cruz, 1:500, overnight at 4 °C), mouse anti-Keap1 (sc-365626, Santa Cruz, 1:500, overnight at 4 °C), rabbit anti-c-Myc (#5605, Cell Signaling, 1:1000, overnight at 4°C), rabbit anti-p62 (ab155686, Abcam, 1:1000, overnight at 4 °C) and mouse anti-beta-actin (#3700, Cell Signaling, 1:1000, 1 h at room temperature) was performed, then the membranes were washed and incubated with an HRP-conjugated secondary antibody (mouse #7076/rabbit #7074, Cell Signaling) at room temperature for 1 hour. .. Imaging of Chemiluminescence signal was performed in a ChemiDoc XRS+ instrument (Biorad).
Article Title: Melatonin receptor activation provides cerebral protection after traumatic brain injury by mitigating oxidative stress and inflammation via the Nrf2 signaling pathway.
Article Snippet: Cite this article as: Junmin Wang, Chao Jiang, Kun Zhang, Xi Lan, Xuemei Chen, Weidong Zang, Zhongyu Wang, Fangxia Guan, Changlian Zhu, Xiuli Yang, Hong Lu and Jian Wang, Melatonin receptor activation provides cerebral protection after traumatic brain injury by mitigating oxidative stress and inflammation via the Nrf2 signaling pathway, Free Radical Biology and Medicine, https://doi.org/10.1016/j.freeradbiomed.2018.12.014
Article Title: A pharmacological modality to sequester homomeric proteins.
Article Snippet: Then, 20–40 μg of each sample was loaded and run on an SDS–PAGE gel, before transferring onto a nitrocellulose membrane using a Trans-Blot Turbo system (Bio-Rad) and blocking using a 5% BSA in TBS-T (w/v) solution for 1 h at room temperature. .. Incubation with primary antibodies (mouse anti-BCL-6 (sc-7388, Santa Cruz, 1:500; overnight at 4 °C), mouse anti-Keap1 (sc-365626, Santa Cruz, 1:500; overnight at 4 °C), rabbit anti-LDHA (4H18L6, Thermo Fisher, 1:1,000; overnight at 4 °C), mouse anti-NQO2 (sc-271665, Santa Cruz, 1:500; overnight at 4 °C), rabbit anti-c-Myc (5605, Cell Signaling, 1:1,000; overnight at 4 °C) and mouse anti-β-actin (3700, Cell Signaling, 1:1,000; 1 h at room temperature) was performed and then the membranes were washed and incubated with a horseradish-peroxidase-conjugated secondary antibody (mouse 7076/ rabbit 7074, Cell Signaling) at room temperature for 1 h. Imaging of the chemiluminescence signal was performed using a ChemiDoc XRS+ instrument (Bio-Rad). ..
Western Blot:Article Title: Inhibition of SQSTM1/p62 oligomerization and Keap1 sequestration by the Cullin-3 adaptor SHKBP1
Article Snippet: .. The following antibodies were used for Western blot: mouse anti-SQSTM1/p62 (D5L7G), Cell Signaling Technology (88588); rabbit anti SQSTM1/p62, ABclonal (A19700); mouse anti-Keap1, Santa Cruz Biotechnology (sc-365626); rabbit anti-SHKBP1, Invitrogen (PA521731); mouse anti-Ubiquitin, Santa Cruz Biotechnology (sc-8017); mouse anti-GFP, Santa Cruz Biotechnology (sc-9996); mouse anti-mCherry, Sigma-Aldrich (MAB131873); mouse anti-Actin, MP Bio (08691001); rabbit anti-FLAG, Sigma-Aldrich (F7425); mouse anti-HA, Covance (MMS-101R); The following antibodies were used for IF: rabbit anti-FLAG, Sigma-Aldrich (F7425); rabbit anti SQSTM1/p62, ABclonal (A19700); mouse anti-Keap1, Santa Cruz Biotechnology (sc-365626); goat anti-mouse HRP, Jackson ImmunoResearch Labs (115-035-146); goat anti-rabbit HRP, Jackson ImmunoResearch Labs (111-035-144). .. Reagents were obtained from the following sources: DSP (Dithiobis(succinimidyl propionate)), TCI America (TCD2473); MLN4924, Cayman (15217); MG-132, Selleckchem (S2619); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Protein G Sepharose, BioVision (6511); Ni-NTA agarose, QIAGEN (133203974); Anti-HA magnetic beads, Thermo Scientific (88836); Anti-FLAG magnetic beads, made in-house from NHS magnetic beads, Nacalai USA (TAS8848N1141-10MG) and Anti DYKDDDDK tag, Fujifilm (012-22383); Lipofectamine 2000, Invitrogen (11668019); RNase A, Research Products International (R21750); Puromycin dihydrochloride, Sigma-Aldrich (P8833); Blasticidin S hydrochloride, 10 mg/ml in HEPES buffer, Alfa Aesar (J67216); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); ProLong Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); Clarity Western ECL Substrate, Bio-Rad (1705061).
Article Title: Cullin-3 adaptor SHKBP1 inhibits SQSTM1/p62 oligomerization and Keap1 sequestration
Article Snippet: .. The following antibodies were used for Western blot: mouse anti-SQSTM1/p62 (D5L7G), Cell Signaling Technology (88588, RRID:AB_2800125); rabbit anti SQSTM1/p62, ABclonal (A19700, RRID:AB_2862742); mouse anti-Keap1, Santa Cruz Biotechnology (sc-365626, RRID:AB_10844829); rabbit anti-SHKBP1, Bethyl Laboratories (A304–891, RRID:AB_2621086); mouse anti-SHKBP1, Santa Cruz Biotechnology (sc-390121, RRID unavailable); mouse anti-ubiquitin, Santa Cruz Biotechnology (sc-8017, RRID:AB_628423); mouse anti-GFP, Santa Cruz Biotechnology (sc-9996, RRID:AB_627695); mouse anti-mCherry, Sigma-Aldrich (MAB131873, RRID:AB_11034849); mouse anti-Actin, MP Bio (08691001, RRID:AB_2335127); rabbit anti-FLAG, Sigma-Aldrich (F7425, RRID:AB_439687); mouse anti-HA, Covance (MMS-101R, RRID:AB_291262); rabbit anti-Tubulin, Cell Signaling Technology (2128S, RRID:AB_823664); rabbit anti-Histone H3, Cell Signaling Technology (4499S, RRID:AB_10544537); mouse anti-Nrf2 (A-10), Santa Cruz Biotechnology (sc-365949, RRID:AB_ 10917561). .. The following antibodies were used for IF: rabbit anti-FLAG, Sigma-Aldrich (F7425, RRID:AB_439687); rabbit anti-LC3A/B (D3U4C), Cell Signaling Technology (12741S, RRID:AB_2617131); rabbit anti SQSTM1/p62, ABclonal (A19700, RRID:AB_2862742); mouse anti-Keap1, Santa Cruz Biotechnology (sc-365626, RRID:AB_10844829); mouse anti-Nrf2 (D1Z9C), Cell Signaling Technology (12721S, RRID:AB_ 2715528); Alexa Fluor 594-conjugated goat anti-mouse IgG, Thermo Fisher ( R37121 , RRID:AB_2556549); Alexa Fluor 488-conjugated goat anti-rabbit IgG, Thermo Fisher (A11008, RRID:AB_ 143165); goat anti-mouse HRP, Jackson ImmunoResearch Labs (115–035-146, RRID:AB_2307392); goat anti-rabbit HRP, Jackson ImmunoResearch Labs (111–035-144, RRID:AB_2307391).
Imaging:Article Title: A pharmacological modality to sequester homomeric proteins.
Article Snippet: Then, 20–40 μg of each sample was loaded and run on an SDS–PAGE gel, before transferring onto a nitrocellulose membrane using a Trans-Blot Turbo system (Bio-Rad) and blocking using a 5% BSA in TBS-T (w/v) solution for 1 h at room temperature. .. Incubation with primary antibodies (mouse anti-BCL-6 (sc-7388, Santa Cruz, 1:500; overnight at 4 °C), mouse anti-Keap1 (sc-365626, Santa Cruz, 1:500; overnight at 4 °C), rabbit anti-LDHA (4H18L6, Thermo Fisher, 1:1,000; overnight at 4 °C), mouse anti-NQO2 (sc-271665, Santa Cruz, 1:500; overnight at 4 °C), rabbit anti-c-Myc (5605, Cell Signaling, 1:1,000; overnight at 4 °C) and mouse anti-β-actin (3700, Cell Signaling, 1:1,000; 1 h at room temperature) was performed and then the membranes were washed and incubated with a horseradish-peroxidase-conjugated secondary antibody (mouse 7076/ rabbit 7074, Cell Signaling) at room temperature for 1 h. Imaging of the chemiluminescence signal was performed using a ChemiDoc XRS+ instrument (Bio-Rad). ..
|
Santa Cruz Biotechnology is a verified supplier 
